미니돼지에서 자가 피부유래 간엽성 줄기세포를 이용한 상악동저 거상술

변준호; 강은주; 맹근호; 노규진; 강동호; 이종실; 박봉욱

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미니돼지에서 자가 피부유래 간엽성 줄기세포를 이용한 상악동저 거상술
Maxillary sinus floor elevation using autogenous skin-derived mesenchymal stem cells in miniature pigs

대한구강악안면외과학회지 2010년 36권 2호 p.87 ~ 93

변준호, 강은주, 맹근호, 노규진, 강동호, 이종실, 박봉욱,

소속 상세정보

변준호 ( Byun June-Ho ) - 경상대학교 의학전문대학원 구강악안면외과학교실
강은주 ( Kang Eun-Ju ) - 경상대학교 수의과대학 수의학과
맹근호 ( Maeng Geun-Ho ) - 경상대학교 수의과대학 수의학과
노규진 ( Rho Gyu-Jin ) - 경상대학교 수의과대학 동물의학연구소
강동호 ( Kang Dong-Ho ) - 경상대학교 의과대학 신경외과학교실
이종실 ( Lee Jong-Sil ) - 경상대학교 의학전문대학원 병리학교실
박봉욱 ( Park Bong-Wook ) - 경상대학교 의학전문대학원 치과학교실 구강악안면외과

KMID : 0355620100360020087

Abstract

Introduction: In our previous studies, we isolated porcine skin-derived mesenchymal stem cells (pSDMSCs) from the ears of adult miniature pigs and evaluated the pluripotency of these pSDMSCs based on expressions of transcription factors, such as Oct-4, Sox-2, and Nanog. Moreover, the characteristic of mesenchymal stem cells was revealed by the expression of various mesenchymal stem cell markers, including CD29, CD44, CD90, and vimentin. The aim of this study was to evaluate in vivo osteogenesis after maxillary sinus lift procedures with autogenous pSDMSCs and scaffold.
Materials and Methods: The autogenous pSDMSCs were isolated from the 4 miniature pigs, and cultured to 3rd passage with same methods of our previous studies. After cell membranes were labeled using a PKH26, 1×107 cells/100 μL of autogenous pSDMSCs were grafted into the maxillary sinus with a demineralized bone matrix (DBM) and fibrin glue scaffold. In the contralateral control side, only a scaffold was grafted, without SDMSCs. After two animals each were euthanized at 2 and 4 weeks after grafting, the in vivo osteogenesis was evaluated with histolomorphometric and osteocalcin immunohistochemical studies.

Results: In vivo PKH26 expression was detected in all specimens at 2 and 4 weeks after grafting. Trabecular bone formation and osteocalcin expression were more pronounced around the grafted materials in the autogenous pSDMSCs-grafted group compared to the control group. Newly generated bone was observed growing from the periphery to the center of the grafted material.

Conclusion: The results of the present study suggest that autogenous skin-derived mesenchymal stem cells grafting with a DBM and fibrin glue scaffold can be a predictable method in the maxillary sinus floor elevation technique for implant surgery.